Dear all,
I would like to know if there is a strategy we can prolong the thermal stability of reduced glutatione at 80 °C?
The main obstacle in storage of reduced glutathione whether in powder or aqueous solution is the autoxidation which converts GSH to GSSG and this conversion increases with temp. Powder GSH can be stored at 2-8 C for months while aqueous need to store in freezing usually -20C. In your case 80C is very high you might try to minimize autoxidation by adding N-Ethylmaleimide NEM new N2 or used Ascorbic acid or ascorbic acid derivatives or N-acetylcysteine to reduce the autoxidation processes
You can decreased the pH and/or add metal chelators.
Take a look at:
Stevens, R., Stevens, L., Price, N., 1983. The stabilities of various thiol compounds used in protein purifications. Biochemical Education 11, 70. https://doi.org/10.1016/0307-4412(83)90048-1
You can also try to use dithiotreitol (DTT) to reduce GSH.
Many protocols used it to reduce GSSG and assess total GSH