Antibiotic resistance , enzyme production are types of screening method . Both have advantages and disadvantages so , in these two which method is more preferred to insert in vector and provides maximum results . Are there any considerations of these methods while performing gene cloning?
Hello,
Sometimes you have no choice and you have to use one method rather than the other, for example when the selection marker is at the host cell level.
When you have the choice, you should know that for the production of enzymes (for example, B-galactosidase), you will directly select the right clone which has the insert, this clone will no longer produce the enzyme (insertional inactivation) . However, when you are going to use the antibiotics, the selection is done in 2 stages, because the right clone with the insert will become sensitive to the antibiotic and therefore die and therefore it must be recovered from another bacterial culture at which you did not add the antibiotic.
Hi there,
Your question is a bit of unclear to me... When you mention enzyme production, do you mean the enzyme produced by the gene conferring resistance or do you mean the product of the gene of interest possibly cloned into the vector?
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