The use of dodecylmaltoside- detergent to purify GLUT 1 from red cell membranes?

More Frozan Ahmadi's questions See All

Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View

I have started to design a Lithium ion battery in Comsol. I need to merge the LIB model with PDEs. any idea how to do that ?

the electrode structure has secondary pore networks , so I need to use different governing equations for the electrodes. I tried to use PDEs , but I have not get any results. Does anybody have any...

02 January 2021 3,225 1 View

How many and which spin multiplicities we should choose for optimization the adsorption of O2 gas with metal complexes?

I want to optimize the interaction between the the O2 molecule and a metal nanocluster, but I need to know which multiplicities I should choose?

25 November 2020 716 2 View

Why Hiden mass spectrometer shows wrong percentages after calibration?

Hello every one I was wondering if someone could help me solve the problem that I have with my Mass Spectrometer. Actually I have already calibrated my MS device for a mixture of 60% Ar and 40 %...

19 November 2020 2,984 3 View

Problem with converting FEM code from MATLAB into Python; what is the fastest solution?

Dear all, I developed a hybrid Phase Field-Finite Element Model (PF-FEM) for fracture of electrode particles of Li-ion batteries. [Isotropic & Hybrid] Phase Field-Finite Element Model (PF-FE......

31 October 2020 8,810 1 View

Dimensionless geometry in Ansys Fluent?

Hi Everyone. I have this dimensionless governing equations that I want to solve in Ansys Fluent. But then, in Ansys Fluent, the geometry only dimensional. So my question is, does dimensionless...

17 September 2020 9,096 3 View

What is the best method for the reaction of a acid and an epoxy monomer?

I am going to react a free fatty acid with 1,2-epoxydecane.

11 August 2020 8,227 2 View

Grow E.coli in 384 microplates without shaking?

Hello, I need to grow E. coli in 384 microplates directly from colonies. They contain an Amp resistance plasmid. We cannot shake the plate. I tested LB, but they grow poorly. Any suggestion to...

11 August 2020 5,386 3 View

How can I optimize a crystal structure?

I want to optimize a crystal structure that contains a mercury atom with six-coordinated (according to the cif file) but in one monomer of this structure only 3 atoms connected to Hg, I have...

07 July 2020 9,523 3 View

Call for the paper in COVID-19?

Covid-19 in Pharmaceutical and Biomedical Research The novel coronavirus SARS-CoV-2 (COVID-19) is a global crisis leading to huge number of economic losses, morbidity, and mortality. This emerging...

22 June 2020 8,015 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Ahmed Mahdi

Good luck.

Nabaz Fisal Shakir Agha

Iagrre with DR. Kou Hayakawa

with my best wishes.

Frozan Ahmadi

Its strange because I observed bands when probing with a commercial glut1 MAb (directed against the cytoplasmic region).

I used the methods previously described in https://pdfs.semanticscholar.org/f722/be20e9ed242ece16ff10287dc084f7da6ec2.pdf