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Questions related from Frozan Ahmadi
I have recently attempted a GLUT 1 purification method with dodecylmaltoside as a detergent to find the specificity of an anti-glut1 monoclonal antibody. However when probed with my MAb there were...
03 July 2018 8,669 4 View
Hi researchers, I wanted to ask if this attached melt curve show an peaks. I mean, I can definitely see a few to the far left but this melt curve was for control. None of the reaction mixes...
04 April 2017 485 3 View
Hi, I just quickly wanted to gain some opinions on this melt curve. I have optimised the qPCR reaction mix by reducing the primer volume (0.1ul, 0.25ul and 1ul) and run it in duplicates. Any...
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Dear researchers, I have attempted to do a gene expression study. The first step was isolate the RNA. I have checked the RNA yield using Nanodrop, which had the following nucleic Acid...
31 March 2017 7,982 3 View