The answer comes from the my manuscript entitled "A Computer-Assisted Therapy: New Methodology of Predict Restricting Enzymes to Replace Mutations Gene Caused Cancers".
This paper suggested Computer-Assisted Therapy CAT as new methodology, this methodology shows an effective cost and friendly therapy methodology of replacement mutated gene comparing with other methods and this therapy methodology is comprehensive in finding target TP53 (tumor protein P53) gene from DNA template, detecting pri-mers, predict a restriction enzymes, etc. Which reduce the problems within biologic lab when implementing the replacement of mutations TP53 gene.