The standard quercitin on TLC plate is showing two spots one at the point of origin and one on top.Why is standard showing two spots?

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Rashmi Tripathi

The mobile phase is Toluene:Ethyl acetate:Formic acid: Gallic acid (4:5:1:1) reutin is showing two spots and in methanol it is not showing two spots.

Shrikaant Kulkarni

If the standard forms two spots means it forms two solute fronts one near the baseline must be due to impurity and the other spot in top is attributed to parent standard quercitin.

Dear Dr. Shrikaant Kulkarni

Sir but in methanol its not showing 2 spots. can we proceed for HPTLC or we need to change the standard.

regards

Thank you Neelutpal for your suggestion. I would be a great help.

Yannick Fongang

It might be due to slight impurities. Did those spots have the same colour under UV lamp? Quercetin is UV active.

You may also do a double migration to see the behavior on your TLC card.

Aman Paul

Happening because solution is not pure.