The primers with high TM temprature

Marjan Jafarpour @Marjan-Jafarpour

19 October 2011 1 4K Report

I designed a primer with high Tm temperature (74, 70)

[I could not find a better primer. In addition, before PCR, I have to do RT-PCR to convert RNA to c DNA]

1. What kind of PCR do you suggest me? Is TD-PCR proper?

2. If yes what temperature would you recommend for star of gradual decreasing of temperature?

Thank you

Kali Rajan

Dear

There is a formula regarding temperature setting for the particular primer , which is based on how many G,C numbers present.Based on that you get some temperature that must be suitable for your research. Moreover the GC content of the primer should be low which may affect the particular fragment replication.There is no need for using a particular type of PCR. You try with normal one.I will send you the same.

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