Cells are cardiomyocytes of C57 mice born 48 hours.We found that there were many small dots in the nucleus of cardiomyocytes, and our PH3 coincided with these small dots. So we want to know what these little dots are.
Thanks！
Check this paper, you may get something useful.
https://www.nature.com/articles/nature10802
It seems like multi-nuclei cells. As you mentioned it belongs to C57 mice born 48 hours, suggesting that these cells are normally growing and multiplying nuclei to increase the density of cells by multiple divisions.
Murad Alahdal Thank you for your help! Because there are more than 4-5 dots in the nucleus of some cells, I don’t think it is multinucleate cells. And there are some views that in cardiomyocytes, polykaryocytes do not undergo mitosis, so it is difficult to explain the PH3 positive result. I look forward to hearing from you. Thank you very much!
Liu x Xx Sure the multinucleate cardiomyocytes do not undergo mitosis in adult mammalian hearts as reported in my papers. However, your cells have been isolated from newborn mice. Therefore, these cells can normally present multinucleation. Please read about the proliferation of cardiomyocytes in the neonatal cardiac tissue.
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