01 January 1970 2 1K Report

What's the minimal U6 promoter sequence to combine with the multiplexed TetOs for the optimal tTA mediated transcriptional activation (which means the least U6 basal leak without the presence of tTA) ?

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Our truncated protein expressions (labeled by the HA tag) in lane 4 and 5 were supposed to be matched with the mRNA expressions in lane of 293FT-4 and 293FT-5. However, the different truncated...

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Why our truncated protein plasmid expressed correct length of mRNA but incorrect length of protein?

Our truncated protein expressions (labeled by the HA tag) in lane 4 and 5 were supposed to be matched with the mRNA expressions in lane of 293FT-4 and 293FT-5. However, the different truncated...

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Why should we prepare protein samples from dicing SDS-polyacrylamide gels for mass spectrometry?

Why should we prepare protein samples from dicing SDS-polyacrylamide gels for mass spectrometry? Does this step the so-called "reduction" in the LC-MS/MS sample preparation workflow? Is this...

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