Yu Yu

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Questions related from Yu Yu

The issues in the primary culturing of iNKT cells for the potential clinical use?

1. Do we need to separate iNKT cells using certain positive markers, like Vα24 TCRα and CD1d, or negative markers, like CD8, before we can use them in a clinical setting? 2. Should the α-GalCer...

30 January 2024 4,373 0 View

Why our truncated protein plasmid expressed correct length of mRNA but incorrect length of protein?

Our truncated protein expressions (labeled by the HA tag) in lane 4 and 5 were supposed to be matched with the mRNA expressions in lane of 293FT-4 and 293FT-5. However, the different truncated...

20 December 2019 3,385 7 View

Why our truncated protein plasmid expressed correct length of mRNA but incorrect length of protein?

Our truncated protein expressions (labeled by the HA tag) in lane 4 and 5 were supposed to be matched with the mRNA expressions in lane of 293FT-4 and 293FT-5. However, the different truncated...

12 December 2019 5,382 7 View

Why should we prepare protein samples from dicing SDS-polyacrylamide gels for mass spectrometry?

Why should we prepare protein samples from dicing SDS-polyacrylamide gels for mass spectrometry? Does this step the so-called "reduction" in the LC-MS/MS sample preparation workflow? Is this...

01 January 1970 2,997 4 View

The minimal U6 promoter sequence for the optimal tTA mediated transcriptional activation

What's the minimal U6 promoter sequence to combine with the multiplexed TetOs for the optimal tTA mediated transcriptional activation (which means the least U6 basal leak without the presence of...

01 January 1970 1,321 2 View