I'm currently collecting references about staphylolytic assay to detect LasA protease activity and elastin congo red assay to detect LasB elastase activity in Pseudomonas aeruginosa, but from the references I've gathered so far, I can't understand why do we need to use different mediums to conduct each assay? For staphylolytic, the PA needs to be cultured in Luria-Bertani broth (a rich medium). While for elastin congo red assay, the PA needs to be cultured in AB medium (a minimal medium). Is there any specific reason behind this?
This is one of the references, but I got more/less the same method for all journals I've read
Article Inhibition of Quorum Sensing-Controlled Virulence Factor Pro...
Please look at the following below links which may help you in your analysis:
https://iai.asm.org/content/iai/62/4/1320.full.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1196266/pdf/0890-04.pdf
Thanks
Hi, dr
I suggest using of real time PCR by designing a suitable primers for LasA and Las B, then you can get the specific quantities of gene expression for these virulence factors. In stead of depending on the classic method of purification for these proteins which take more time and cost
Dear Muh,
We've used Congo red in the past to investigate cellulose and attachment-factor expression in a biofilm-forming mutant of Pseudomonas fluorescens SBW25 known as the Wrinkly Spreader. Using Congo red with LB medium is problematic, because the NaCl caused the dye to aggregate rather than diffuse evenly through the agar. We use Congo red in Minimal media, in LB (with no NaCl), and in King's B medium (developed for fluorescent pseudomonads).
Regards, Andrew
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays