RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Cross-country research: How can I control for the factor country, although I don't have any hypotheses regarding country differences?

Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...

28 January 2021 5,763 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

What are your tips to write the discussion section of your scientific work in an efficient way?

Writing the discussion section is always the scariest part of a paper or a thesis. I came up with some ideas to make it less difficult, but would like to hear other tips! Here are my two bits:...

04 November 2020 6,808 3 View

How can I determine the total antibodies in the serum of bovines with ELISA?

I want to determine if there is a difference in the amount of total antibodies in the serum of different bovines, I was thinking of doing it with Elisa sandwich. Has anyone tried it? What initial...

13 August 2020 2,412 4 View

What measures have your labs/ institutions put in place to mitigate the impact of COVID-19 on early career-researchers?

Early career-researchers are disproportionately affected by the COVID-19 pandemic in academics, as they often struggle with precarious working situations and they are seeing many doors shutting...

27 May 2020 1,440 1 View

Avigan ( Favipiravir), Hydroxychloroquine plus Azithromycin. A possible Cocktail against SARS-COV2 ?

All the three medicaments have promising results reported and supposed complementary mechanism of action?

10 April 2020 6,909 7 View

DIANA FEA 10.2: How do I model deformation supports, so that no tension occurs/is allowed ?

Hello together, I am working on concrete direct shear Tests, where i apply loadsteps of a prescribed Deformation (left deformation). Since i can only apply a Deformation with a support inplace i...

20 January 2020 8,225 3 View

Is HSE06 a good functional?

I'm investigating first row transition metal oxides on diamond surface, optimizing structures and calculating electronic properties (e.g. electron affinity). I was advised that since I'm working...

08 January 2020 8,713 4 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Desalting using DSC- 18 SPE columns?

I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...

01 February 2021 9,076 1 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

What could be the impediments to the initiatives adopted by the bank towards FI in the Indian scenario?

this question from case Union Bank of India: Initiatives towards IT-enabled Financial Inclusion

09 December 2020 7,721 3 View

How to conduct CUSUM and CUSUMsq test after the inclusion of an exogenous dummy variable in my ARDL model?

Hi all, I have been struggling a great deal with this matter and as such I hope you could provide me with answer. I have added a dummy variable to my ARDL model to improve its stability (Eviews)....

01 December 2020 1,071 5 View

Why Porphyrin is purple in color?

Porphyrin solid is purple in color, what factors are responsible for purple color of the porphyrin?

29 November 2020 199 2 View

What should be the ideal solvent for meso-tetra(2,3,4,5,6-pentafluorophenyl)porphyrin?

I've tried using Acetonitrile but the solubility is very low.If there's any cited articles that'd be very helpful!

22 November 2020 6,929 3 View

Why the expressed protein band Molecular Weight (MW) is lower than expectation?

I tried to express my protein in BL21(DE3) bacteria. The desired protein MW is 65.5 KDa and I know my protein will be in an insoluble form (inclusion body). I separate soluble and insoluble...

20 October 2020 3,975 8 View

How can the inclusion of agriculture in carbon markets provide significant benefits for farmers?

What are the possibilities and implications in this regard

19 October 2020 3,701 5 View