The heated lid on an ABI 2700 PCR machine has broken and stuck in the shut position. Is there an easy/efficient/any way to open it?

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Why are reaction forces so high when I add vibration to cutting model?

Hi I am trying to model a cutting problem with a blade and comparing the results with and without ultrasonic vibration. From experimental results in our group, the use of ultrasound can...

13 June 2024 7,461 4 View

Best Tools for Wrist PPG Signal Analysis: Your Recommendations?

I'm conducting research on photoplethysmography (PPG) signals obtained from smartwatches worn on the wrist. The main goal is to analyze the PPG waveform and extract key fiducial points and...

13 June 2024 3,286 0 View

Why diagonal terms in Abaqus stiffness matrix are infinite?

I am running some simulations in Abaqus to extract the stiffness matrix of a FE model with geometric nonlinearity. I want to provide to Abaqus a set of nodal displacements (all the dofs in the...

28 May 2024 4,797 2 View

Is it possible to combine a time-series of tree-ring widths and and a time-series of diameter at breast height measurements into one TS?

Hello together! I have some time-series for tree-rings from dating from 1850-2016 and diameter at breast height (DBH) from 1996-2022 for the same plot, however the sampled trees for tree-rings...

16 May 2024 5,295 2 View

Is priori knowledge only instincts? Why?

I think so because The Random Slate Theorem

03 May 2024 3,082 7 View

How to fix driver conflicts of an ancient Skyscan 1072 micro CT with DT3157 video grabber ?

I am currently trying to get a Skyscan 1072 micro CT to work again. After the mainboard on the original PC died, we tried to set the machine up on a new computer. We managed to control the source,...

28 April 2024 381 0 View

Statistics: Is it worth using a t-test on pre and post survey data with 12 or less responses?

Is it worth using a paired t-test on pre and post survey data with 12 or less responses? Or should one rely more on the mean and standard deviation of the two data sets to evaluate the survey...

27 April 2024 8,703 8 View

What about religion, God, multi verse, etc.?

1)Religion(or at least spirituality) calms my mind, particularly, the built in belief of only one reality. God created the only reality. The afterlife is another dimension. We have free will but,...

25 April 2024 10,055 0 View

Handling BrainVision matlab files with ECG data in EEGLAB?

I have 217 preprocessed BrainVision matlab files (.mat) with ECG data in them. I've been having trouble creating a study for these. I know BioSig works for ECG data, but it doesn't seem to work...

21 April 2024 1,028 2 View

EEGLAB - how to import multiple .mat files from different folders all at once?

I have 25 folders for 25 participants, all with .mat files inside that are preprocessed. Most of them have the same amount of .mat files - a couple participants didn't finish the study, so they...

17 April 2024 1,897 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View