The currently known methods for detecting DNA damage, such as the comet assay, are generally applicable to all DNA. I would like to inquire whether there is a specific DNA detection method for a particular gene? Thank you very much for answering my question！！！
Hello Chenlong Wang
PCR-based methods may be used. DNA breaks and lesions may be detected by PCR. DNA amplification is stopped at the sites of damage via blocking of the progression of Taq polymerase, which results in a decrease in the quantity of PCR product and a reduced number of DNA templates, which do not contain the Taq-blocked lesions as they are not amplified.
You may want to refer to the articles attached below for more information.
Article PCR-Based Methods for Detecting DNA Damage and its Repair at...
https://www.sciencedirect.com/science/article/abs/pii/S0160932797856669?via%3Dihub
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