I'm interested in click chemistry as a useful tool for formation of cross-linking polymers.

I applied the technique to prepare a cross-linking polysaccharide and got one successfully.

I noticed a cross-linking velocity of the preserved solution (4 ℃) was slow compared with that of the solution freshly prepared.

Although structural stability of both of DBCO- and azide-labeled polysaccharides was checked by using NMR and HPSEC, I could not find any difference in the preserved samples.

I don't know what has happened.

Please help me to solve a problem.