i am studying evolution of family Apiaceae and the relation between phylogeny and secondary metabolite production . i used 13 plant from apiaceae can i use Lactuca sativa and Solanum lycoperiscum species from Composete and Solanaceae familes respectively as outgroup taxa ?
Hi Dalia,
A rule of thumb: "outgroups closely related to the ingroup species are better than distantly related outgroups" (Yang 2006 Computational Molecular Evolution). Thus species from other family of the same order are preferred.
Cheng-Min Shi regrettably , i already used Lactuca , Solanum as cultivated plant and i had a point of view, to study evolution i studied genus from primitive order and advanced order . then i haired that it not correct , what can i do ? i hope i found a reference like that use not close outgroup
Outgroups are closely related so it could be from the same genus with close related groups so that you can study evolution as well as genetic diversity between them. Also u can use mt-DNA of specific individuals so that you can study genetic diversity which can be correlated with evolution.
Dear Dalia
I agree with Chengmin Shi , you can include species belonging to another family from the same order. But, that family should constitute one of the deepest branching lineages within the order.
Best regards
Ania
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