Anyone know the ways I can put microorganism on a stable dormancy state and wake them after some time? But I want to make this on tropical environment conditions(can be in vitro), without freezing them.
Hello, for more reliable answer more details needed.
1. how long you want to keep microorganism?
2. type of microorganism spore-forming or not?
If it spore-forming microorganisms just grow it in tube with media and wait till sporulation after you can keep it in any conditions for years.
If it not spore forming you can use reseeding of microorganisms once a
Two weeks - 1 month, made glycerol stocks as already said above or you can perform lyophilisation and keep again for years.
Good luck!
Hello Samuel,
Drying is one of the another process we used particularly lyophilization (freeze- vacuum drying) as we encapsulated microbes but the time span again was limited. A paper on plant microbes maintenance I came across that can be helpful to you for understanding and choosing the method accordingly.
Article Maintenance and assessment of cell viability in formulation ...
Hopefully this helps you!
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