You can try with 4% (v/v) glycerol or Antifade mounting reagent from Invitrogen (life technologies).

Hello, Do yo mean mixing cell suspension directly with glycerol or antifade reagent and then put a drop over a slide? Doing that way, do bacterial cells stay immobilized? Thanks

To immobilize the cells, antifade reagent shows better results than 4% glycerol, Still you can use it. After sample preparation, give 40 microlt of sample and 10 microlt of antifade reagent on a slide, mix it and place a 22mm sq cover-slip on it. For glycerol, maintain a proportion of 50-50 or 60-40 for sample and glycerol respectively. I follow the same for fluorescence and confocal imaging. Best of luck !!

Thanks!, will try it.

Hi Andres

I have a problem like you. Did you do the recommendation of Debaprasad Parai? and could you observe bacterial cells by fluorescence or confocal microscopy through this method?

Thanks.

Hi,

Try to use a sds page glasses to make 1mm agarose slide. Than you need just to drop 3-10ul cells suspension and let it air dry. I cover it by cover glasses and observe with agarose layer under the object.

Cheers

Hello Sir Dariusz Nowicki ,

I am learner in this field and want to study the fluorescent protein expressed in bacterial. Can I use this technique to immobilize the bacteria?. Thanks