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Questions related from Andres Esteban Marcoleta
We are trying to use a gentamicin resistance cassette as a selectable marker in E. coli and Klebsiella. The cassette was PCR amplified from the CRIM plasmid pAH153 described originally by...
06 June 2014 7,742 0 View
We are measuring gene expression from cDNA samples prepared from bacterial RNA. Before cDNA synthesis, we treated RNA samples with DNAse to remove putative contaminating genomic DNA. However, when...
05 May 2014 3,203 2 View
People often says that plasmids become unstable if you use them to clone long DNA fragments (over 20 kb), and that for this purpose is better to use cosmids (can carry DNA fragments up to 50 kb)....
04 April 2014 7,201 5 View
I'm trying to use qRT-PCR to determine the copy number per chromosome of a problem DNA in a sample. I amplified my problem DNA and got a Ct of 20, in parallel I amplified a rpoD gene that is in 1...
09 September 2013 6,781 7 View
Several works discuss about which housekeeping genes are more suitable to be used as endogenous references for normalization in eukaryotes. However, to my best knowledge, no similar comparisons...
08 August 2013 1,846 8 View
I'm trying to run the tRNAcc software to identify tRNA-inserted genomic islands in several bacterial genomes. However, it seems that tRNA genes must be annotated in a specific way to be recognized...
08 August 2013 247 4 View
I tried several mounting alternatives to observe bacterial cells by fluorescence microscopy but none of them made me happy enough. Now, we are just embedding glass slides with 1% agarose in PBS...
07 July 2013 5,976 7 View
