How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Could you please share your experience in learning SPSS?

I would like to learn SPSS. Could you please guide me or share your experience if you feel it would help me to learn SPSS? Thank you very much indeed!

08 November 2020 7,357 6 View

How do I compare time domain solution with frequency domain?

I am solving EM wave scattering by PEC cylinder numerically and wanted to compare the scattering width. My result is in the time domain which is evolving with time but the analytical solution is...

11 October 2020 770 3 View

Is it possible to consider intermittency of renewable energy sources by using normally (Gaussian) distributed random numbers ?

Consider wind speed for wind power and solar radiance for solar power. Is it possible to consider intermittency of mentioned sources using normally ( Gaussian ) distributed random numbers in which...

29 January 2020 5,731 3 View

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

12 January 2020 9,036 3 View

How to prepare 1U of lyophilized powder of Bacillus stearothermophilus?

First of all, i have narrow knowledge about antidiabetic assay due to this is my first enzymatic test and i have no one to refer. I have a method but when i tried to google every each of the...

05 January 2020 4,629 3 View

How to compute unknown parameters in an equation from experimental data?

Is there a way to compute some unknown parameters in an equation using curve fitting using experimental data? I am upload a picture which contains the function and three unknown parameters and...

25 December 2019 2,184 7 View

From the Table attached, why Retentivity is decreased for Bi:ZnO 3% and for Bi:ZnO 5%, Coercivity is decreased but Ms is increasing linearly for all ?

Hi all, referring to the table attached, it is seen that when ZnO NWs are doped with Bi at different concentrations, there is an increase in magnetization values initially. But we see by doping...

08 October 2019 3,938 3 View

How to make 500 unit/ml catalase solution using catalase protein from Sigma?

13 August 2019 7,931 3 View

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10 March 2019 8,910 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View