Hello Sandra,
However, I do not know the answer of your question, I found myself have to comment on the genetic background that you mentioned. The C57BL6J mice lack the Nicotinamide nucleotide transhydrogenase (Nnt) gene. The Nnt gene is important for glucose homeostasis and control insulin secretion. You can read more about that in the following article https://www.ncbi.nlm.nih.gov/pubmed/19448337.
I may advice you to use another C57BL6 background for instance C57BL6N.
Good Luck!
Mahmoud
For sure it will depending also on fat diet nature.
https://www.ncbi.nlm.nih.gov/pubmed/22166251
https://www.ncbi.nlm.nih.gov/pubmed/11882503?dopt=Abstract&holding=npg#
Dear Sandra I suggest that you read this article and similar ones. This will help you find the answer to your question.
We usually use 4-week-old C56BL/6J mice and feed them high fat diet (60% calories from fat, prepared by Research Diet Inc.) for 12 weeks. The mice then become obese and develop hyperglycemia and insulin resistance.
Dear Sandra, according to our lab experience, we usually use 4-week-old C57BL/6 mice and feed them high-fat diet (composed of 60% fat, 20% carbohydrate, and 20% protein based on caloric content), Research Diet Inc, USA, catalog number D12492 for 12 weeks. As a result, our mice then become obese and develop hyperglycemia- insulin resistance. I think after ten weeks should be drawn up to obesity and insulin resistance. Hope can solve your problem. Thanks
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