Tetra-Arms PCR: Gel shows CC and CA alleles but no AA alleles in any sample. Technical issue or probability?

05 July 2023 1 6K Report

40 samples show one heterozygous (CA)(175bp, 202bp) and one homozygous allele (CC)(202bp) only and not (AA)(175bp) allele. Attached is just a snippet of some samples showing CA and CC allele mutations. Outer primers cover band size of 326bp.

2.5% agarose gel electrophoresis. Longitudinal. Long run.

Katie A S Burnette

You'll have to provide some more background.

What genotypes are you expecting? In what proportion? What is the sample population (mutagenized? inbred? wild? etc.)

You can't involve probability unless you have expected numbers to start with.

Also, do you have controls to show that AA genotype is detectable with your assay?

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