Mishal Shaheen

4 Questions 2 Answers 0 Followers

Questions related from Mishal Shaheen

What is the Optimum Neon Transfection Setting for CRISPR/Cas9 RNP in THP-1 Cell Line?

I am trying to transfect THP-1 cells with RNP of CRISPR/Cas9-GFP using ThermoFischer Scientific Neon Transfection System. The current settings are 1400V, 20ms breadth, and 2 pulses. I have done it...

27 May 2024 944 0 View

Tetra-Arms PCR: Gel shows CC and CA alleles but no AA alleles in any sample. Technical issue or probability?

40 samples show one heterozygous (CA)(175bp, 202bp) and one homozygous allele (CC)(202bp) only and not (AA)(175bp) allele. Attached is just a snippet of some samples showing CA and CC allele...

05 July 2023 5,689 1 View

Tetra ARMS PCR shows only one mutation in samples. How to troubleshoot to get the other when I don’t have any Positive control?

My Outer forward, Outer Reverse bp size is 326bp, which shows in my samples. There are two possible mutations: One showing band size of 202 and the other at 175. All of my samples show either no...

09 June 2023 227 7 View

Slow Haddock 2.4!

Has anyone faced slow HADDOCK 2.4 version? My job is running since 48 hrs!

01 January 1970 8,965 0 View