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I believe that attB sites are on your e.g. PCR product. Which permits them to be dropped into a DONR plasmid containing attP sites (and a ccdB cassette), creating an ENTR plasmid (contains your gene, has ccdB removed) for the LR reaction with a DEST (destination) vector, resulting in your expression vector, that will be used for whatever your application actually is. This is how we do it in my work flow.
Thank you for your answer :) That's what we usually do, too
I just wonder if the way I mentioned would work, too.
Oh, sorry. I miss understood your objective. :) I would expect any attB site harboring construct to transfer to a P site harboring one. I have not done that, but, I would expect it to work.
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