25 January 2020 3 3K Report

For writing grant proposal as a part of coursework, my objective is to find out methylation status of 3 genes in Pseudomonas aeruginosa and staphylococcus aureus

More Prajakta Biyani's questions See All
Which approach is better to avoid pressure surges occuring in case of extreme valve position changes?

The existing control system for the fluid flow control consists of a cascaded PI controller with inner (faster) PI controller for controlling valve position change and outer (slower) PI controller...

12 January 2016 4,682 3 View

Similar questions and discussions
Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

P53 detection in methylated and non methylated samples?

Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...

04 February 2021 2,749 3 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can someone explain why a methyl in phentolamine mesylate presents two correlations for 13C-HSQC, around the region it should appear?

Instead of appearing one single correlation of the signal of the carbon at 20.9 ppm (#11 in the attached figure), aligned to hydrogen at 2.29 ppm, it appeared two correlations (2,32 and 2,26 ppm)....

16 January 2021 4,774 2 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close