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Questions related from Morgan Sobol
Hello all, I was wondering if there is a negative effect of RNase inhibitors (like RNase Out, RNasin, etc) on low input RNA samples at higher concentrations? I find that many single-cell RNAseq...
20 October 2021 6,034 2 View
Hello, From my understanding, there are two ways to add polyA tails to RNA. 1) with polyA polymerase (PAP) and ATP to RNA or 2) with terminal transferase (TdT) plus dATP and ddATP to ssDNA...
19 October 2020 6,311 2 View
Hello, I have been trying to find studies that have FACS sorted E.coli in bulk for RNA extraction and RNA-seq. Maybe I am bad at lit-searching, but I have been surprised to not find a clear...
15 November 2019 4,843 3 View
Hello everyone, I am trying to use a more accurate cell counting method than the traditional plate count method. Based on these two papers...
19 July 2019 1,482 6 View
Hi, I am trying to put together a list of the methods used to determine how much mRNA you have after RNA extraction. My plan is to compare total RNA vs. mRNA amounts before and after rRNA...
26 April 2019 8,104 2 View
I measure the dry weight biomass of fungi overtime. Sometimes the growth curves are not perfect and determining a growth rate from the log/exponential phase is difficult. What suggestions do you...
07 December 2017 4,796 2 View
I am looking for a protocol that will allow me to measure nitrite and ammonium in liquid fungal culture spectrophotometrically. I already have an idea of the chemicals I will use and a general...
06 April 2017 6,184 3 View
Hi, I am looking for an alternative for Tween 80, 20 as well as Triton X-100. I have none of these chemicals. Thanks!
02 June 2016 1,424 4 View
I am growing Penicillium chrysogenum and Penicillium brevicompactum in a liquid batch of potato dextrose and streptomycin. I am trying to make them sporulate. By increasing the incubating...
27 May 2016 8,143 4 View
