Why do we add urea with ammonia metavanadate for making of vanadium pentoxide
Urea is added to control pH and ammonia metavandate is the source of vanadium species
How to conjugate two acids with amine linker?
18 February 2021 4,083 2 View
Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...
16 February 2021 1,389 2 View
Hi everybody, currently, I am working on the grid-connected inverter. I want to make my grid-connected PV system a PLL-less system. I have studied some reachers are implementing a virtual...
07 February 2021 6,078 2 View
Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India
04 February 2021 8,632 8 View
If condensation reaction happened between carboxylic acid and amine and amide is formed. The solvent for this reaction is toluene. After completion of reaction, how to collect product? It should...
03 February 2021 9,796 3 View
Hey, I want to know if I have fabricated some nanostructure and further I want to make a shell on the nanostructure by solution phase method by making it core-shell nanostructure. So, anybody can...
01 February 2021 9,966 4 View
My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...
30 January 2021 1,039 3 View
The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...
18 January 2021 5,015 3 View
Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...
10 January 2021 6,346 6 View
Dear Research Gate Members, I have a query regarding comparing the two groups on the basis of the regression models For Instance: I have two sector companies i.e. Public Sector and Private...
09 January 2021 9,645 8 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Is there any heat of dilution which I need to take care by putting some circulation cooler? Will there be any significant ammonia emission in the process?
09 February 2021 5,943 2 View
Hello, I synthesized PEG coated Fe3O4 nanoparticles by coprecipitation methods and I would like to ask whether they can be dispersed in aqueous NH3 since they have a PEG coating and theoretically...
06 February 2021 5,217 4 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
I have to develop a method that can be good enough to run dozens of samples that include urea as the main compound. I prefer an HPLC method because of the convenience of running large numbers of...
01 February 2021 1,888 4 View
Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...
27 January 2021 4,055 4 View
Hi everyone! I`ve question about vanadium pentoxide. Exactly about precursors for the synthesis, about conditions. For example I need to obtain V2O5 powder, should I firstly obtain NH4VO3, or I...
11 January 2021 1,382 4 View
Hi everyone, I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:...
07 January 2021 8,023 3 View
Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.
04 January 2021 7,357 1 View