We are basically forming the silver nanoparticles by bioreduction methods by using flavonoids as source. we have extracted our polyphenolic flavonoids in ethanolic extract from plants and we are making their stock solution of extracts in 50% ethanols. We have used different methods to synthesis such by adding 1ml of extract in 10ml of 1mM of Silver nitrate but after 24hrs , wevare not getting any king of curve in UV-Vis scanning but while going for DLS we are getting size more than 300nm.
Instead of that i have also prepared by just heating silver nitrate at 80 degree C solution and stirring with magnetic beads and adding drop by drop extract in large amount so that brown colour can be observed to see the step wise reduction but still the size is getting high in between 300-600nm that was after sonication.
I am not able to understand. Even what i had done today i have centrifuge the the synthesize nanoparticle and then and then re submerged the pellet in deionized water, after that i have observe the UV-Vis spectra scanning over their i got two curves one at 250nm wavelength and other at around 360nm. what does it mean ?
That should i go for for DLS after purification or before ?
What should be the size of bioactive compound loaded nanoparticles ?
You can reduce the amount of plant extract from 1ml in 10ml of Silver nitrate to 0.5ml in 9.5ml. And one more suggestion is that kindly check the preparation of 1Mm Silver nitrate. If your preparation is not correct, you will find the same problem again.
Hello Yash,
Have you checked the pH of your solution? Will you be doing optimization of different parameters? I am in the process of optimizing parameters for AgNP synthesized from plant extract. I have tried mixing my extract and silver nitrate solution first before heating it for 15 minutes at 80 C... DLS results showed an average size of 55 nm...
Thank u sir, but I cannot reali on dls as it give the broad range not exact. the protein conjugates I have formed it was coming in dls, approx 110nm but in AFM it was observed 14nm size silver nanoparticle
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