I am going to use the abcam sumoylation kit ab32518, and I was wondering if someone ever used it with cellular extracts cottoning a putative E3 SUMO ligase. IF so, how much input lysate (concentration) should I use?
Hi, I'm a masters student struggling with the design of my cognitive psychology project. We wish to run an experiment where we examine false memory recall using a list of schema related nouns in...
13 February 2021 6,457 3 View
Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.
28 October 2020 8,773 3 View
Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...
23 September 2020 3,849 1 View
Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...
12 August 2020 5,382 2 View
Hello, I'm trying to customize a CCA biplot made with the plt.var function from the CCA package. I need to reduce the size of the labels or be able to move those labels because they overlap on...
31 July 2020 2,938 3 View
There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...
13 July 2020 7,807 12 View
Imagine SARS-COV-2 in a surface to be recovered with an hyssop .
08 July 2020 1,193 3 View
I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...
06 July 2020 9,618 1 View
I think it would be interesting to implement the propagation of the COVID-19 virus in some simulation software for the circulation of people, for a population diverse in ages, heights, etc.,...
30 June 2020 7,481 14 View
It should be mandatory to learn from what life teaches us. With COVID-19, we have been forced to buy sanitary materials because in our country there was no industry to do so (dependency) and we...
30 June 2020 3,018 36 View
I wish to simulate a scenario in SUMO as follows: I wish to define some vehicles that are randomly located in a residential area on the network, and then these vehicles will move to their...
25 November 2020 3,196 2 View
As far as I know, the same plan might create different events when executed multiple times in MATSIM. But, I am looking for a way that whenever I run a particular scenario for my agents, the...
13 November 2020 8,544 1 View
I am new to these network simulators (OSM, SUMO and NS2), but need to use the simulators for a project i am currently working on. i am total a beginner at these simulators, therefore, i will...
28 August 2020 2,734 5 View
Dear Experts, I have executed Ant colony algorithm,which gives optimal route in terms of trail of junction of node. ACO considered parameter of Distance and Pheromone. Now how to update this...
16 December 2019 5,245 3 View
Hello friends, I am a beginner in urban traffic simulation. I searched on the internet about traffic simulators in days ago. I am familiar to Matlab and I found that Matlab can be used in VANET...
18 July 2019 8,431 2 View
Hi everyone, I have to use the SUMO protease to cleave the SUMO protein from my recombinant protein. After that I will put in contact (ice, one hour) the product of the digestion with the resin...
11 June 2019 6,599 1 View
I am trying to digest a pET-11a SUMO vector with Kpn1 and BamH1 (expected weights of both bands approximately 5770 bp and 210 bp, respectively), but I am getting mixed information on the NEB...
05 June 2019 8,555 2 View
05 June 2019 3,122 1 View
My peptide has strong interaction with SUMO, when I use 10 kDa molecular cut-off, the peptide also can not come through, just ask for help that any solution can break the interaction between...
03 April 2019 6,252 1 View
Dear All, Any Idea on How to implement Ant Colony Optimization with SUMO + Traci? I stuck badly. Please help me to save my life.
31 December 2018 2,541 4 View