Dear colleagues! Who of you used SR101 in your practical work? Please, describe your experience in a few words. Did you face with unspecific staining, toxicity? Thanks.
Dear all! I want to install some markers into lateral line of zebrafish. May be, any of you did this? Do you have any cell line haveing properties to zebrafish neuromast which could be a model in...
10 November 2018 1,505 0 View
Dear colleagues! I want to know any approach, technique, compound including any polymer which will improve mechanical stability of outer hair cell in electrophysiological experiments where these...
10 November 2018 1,484 0 View
Dear colleagues! Could you advice me any cell line which I can use as a model of OHC (outer hair cells)? HEK is widely used but I think to test other one. Thanks. Tatiana
09 October 2017 7,556 0 View
Dear colleagues! Could you advise me any journal where we can publish results of work deals with collagen gel chemical stabilization? Thanks.
08 September 2016 1,875 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View