Greetings!

I hope you are all doing well. Lately, I have been working on tripolymeric triaxial nanoparticles (PCL as core, gelatine as intermediate and PLGA (85:15) as shell). I have successfully managed to achieve optimized parameters for core and intermediate layers for electrospraying but for gelatine, I have some difficulties. I am looking for an alternative solvent, in order to use instead of water. I have tested a variety of options;

I knew it could be dissolved in acetic acid. So I went for the acid, took a lot of time, even at elevated values below degradation temperatures. Results were not satisfying. Furthermore, I worked on preparing a solvent system, combining acetic acid and ethyl acetate together, with different proportions, did not work as well!

I might go for DMSO (considering its cytotoxicity) and PEG. I would really appreciate it if someone could aid me in this regard.