You can use program available in Expasy tools (free online)

Or for hydropathy plots at the membrane interface you can use : the program MPEX available online at: blanco.biomol.uci.edu/mpex/

Thanks alot, Stefania,

I have chosen to use expasy tool (http://web.expasy.org/protscale/), I am having few glitches, as I would want to use hydropathy plots as a mode of comparison of 7 different proteins sequence composition, which i plan to do so by overlapping the plots of 7 proteins in different colors, I did try it in expasy, but i am able to get one plot at a time.

So is it possible for me to upload 7 different sequences at once to get 7 plots in the same graph.

Kindly advice.

I usually use MPEX for getting graphs. But I imagine that also from expasy you can get the data points and make a superimposed graph with Excell. Hope it is usefull. Stefania

I think i can manage with MPEX as its simple to use.

Thanks alot :) :)