suggestions for using alternate pcr method for race

26 July 2011 1 4K Report

hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..

thank you sir! but before going to transformation by agrobacterium it need to amplyfy cDNA and put into cloning and expression vector, iam asking alternate amplification pcr method instead of race..

Badges
Science method

More Nagaraju Marka's questions See All

How can i start study on warm whole theory?

To study the warm whole theory, what is the roots of the this theory and where can i start and which books will i prefer.

26 April 2024 2,788 2 View

Which are Quick Publishing Scopus Journal in the area of manufacturing and composite materials ?

I am working on Aluminum based metal matrix composite. i would like to publish my work in Scopus databased but I have limited time to get publication. please let me know which is the best and...

21 January 2024 4,653 1 View

Asking about Enrichment ratio in Hirshfeld surface analysis?

Hello Researchers, Can anyone please answer, how to do the enrichment ratio in hirshfeld surface analysis? With regards Maithra N. Research scholar

25 December 2023 5,405 4 View

How to install Schrodinger desmond maestro?

how to install academic free Schrodinger desmond maestro software in Linux to run dynamic simulation?

11 July 2023 7,335 6 View

Which software is suitable to simulate IC engine fuel consumption and performance?

Hello I would like Investigate whether Metal Matrix composite material is suits for IC engine connecting rod. In this connection i would like to conduct performance (Fuel consumption) test on IC...

13 November 2022 5,644 4 View

Which digital controller is suitable for unified power quality controller ?

for upqc controller strategy implementation

16 March 2022 8,627 3 View

I have an idea of to combine the prismatic compass and surveyears compass we take readings of FB & BB in one instrument but how to proceed ?

have an idea of to combine the prismatic compass and surveyears compass we take readings of FB & BB in one instrument but how to proceed ?

11 March 2022 9,840 0 View

How to analyse taguchi design data using ANOVA in minitab ?

Dear all I designed the experiments using Taguchi software, But I am unable to analyze the ANOVA analysis please help me in that. Thank you

11 May 2020 4,950 0 View

How exactly reverse current braking works on BLDC motor? how fast we can stop motor with this method?

I wanted to stop the BLDC motor with reverse current braking with time less than 10ms. Is this feasible?

01 September 2019 4,805 3 View

Can anyone send EVAPOTRANSPIRATION global Monthly data download link?

EVAPOTRANSPIRATION global Monthly data download link from 1980 to 2018.

05 May 2019 1,061 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View