hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..
thank you sir! but before going to transformation by agrobacterium it need to amplyfy cDNA and put into cloning and expression vector, iam asking alternate amplification pcr method instead of race..
i am planning to do research on the topic " Social media as a Source of Information to Engineering Students" for this please share valuable thoughts.. which type of Hypotheses can suitable for...
27 October 2018 8,864 3 View
For a Mathematics Postgraduate, who is interested in doing his PhD in areas related to Algebra and Analysis, what research field would you suggest. Preferably, the domain should be promising and...
31 January 2018 4,612 4 View
Dear All, I have identified a putative male sterile line in okra(Abelmoschus esculentus- through wide hybridization) (confirmed sterility with crossing/ selfing/ pollen studies). In order to...
10 October 2017 7,706 3 View
Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?
20 December 2016 2,182 3 View
I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...
26 January 2016 4,477 2 View
electrical engineering
11 February 2015 6,989 3 View
what are the different reducing methods and reduction chemicals...
24 August 2014 2,993 3 View
I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...
21 August 2013 4,011 16 View
I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...
20 June 2013 2,588 2 View
I have been trying to prepare root tips for chromosome counting studies in okra (Abelmoschus), but every time I am failing to get stained root tips with Feulgen stain. The root tip portion is not...
01 January 1970 5,105 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View