We need to identify specific neuronal cell types in the cerebellum of rat and mouse using immunolabeling approaches. A number of markers have been identified that can be used to selectively label Purkinje cells (Calbindin, for example) and NeuN is reported to be a marker for cerebellar granule cells. We are interested in markers that could be used specifically to distinguish basket cells, stellate cells, Golgi Cells, and Lugaro cells (all of which are GABAergic and could be labeled for GAD, but that wouldn’t differentiate among these four cell types). Markers that could be used to distinguish amongst granule cells and unipolar brush cells (which are both glutamatergic) would also be helpful. Similarly, if you know of IHC markers that can be used to identify specific sets of synapses made be these various cell types, I’d love to know about it (VGluT2 is a specific marker for climbing fibers. VGluT1 should identify the parallel fiber terminals and mossy fiber inputs).
Any input on specific markers or literature references that show marker labeling for these various cell types would be welcome. Thank you.
Dave Sherry
It is really very difficult to distinguish these cell phenotypes using simple immunostaining.
Lugaro cells, I know it’s also serotonergic.
Microinjection of AAV-specific promotor-fluorophore into cerebellum, maybe helps.
Pax6 - Granule cells
Tlx3 - subpopulation of granule cells
Tbr2 - Unipolar brush cells
Foxp2 - Purkinje cells
Pax2 is a general cerebellar interneuron marker.
Hope this helps!
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