Can I use a hot-start Taq for pre-selective PCR in AFLP?

I'm trying to run a methylation-sensitive AFLP analysis and I only have hot-start taq (GoTaq). I've seen in other AFLP procedures that you should not use hot-start Taq for pre-selective PCR...

06 July 2017 6,606 4 View

Can I use a tabletop centrifuge (max 3000rpm) with Qiagen's DNeasy 96 Blood & Tissue kit?

I see in the Qiagen handbook that it recommends you buy their proprietary centrifuge in order to get high enough rpms (3000 & 6000) necessary for optimal DNA extractions. I have access to a...

06 July 2017 1,649 2 View

What does a "5% ramp down to 57C" mean?

The PCR protocol says after 15min at 95C, there was a 5% ramp down to 57C. I'm familiar with a touchdown PCR, but this terminology is new to me. Does this mean the annealing step starts at 95C and...

01 February 2016 9,628 3 View

Is there a tool to help design primers for single base extension?

I'm trying to design primers for analyzing SNPs via single base extension. I know I need to use a F or R primer that lines up to the SNP, but I'm trying to figure out how many base pairs to use,...

08 September 2015 1,872 3 View

Can you give any tips for designing primers when there is no flanking region?

We are trying to design primers for SNPs that have little to no flanking region on the 5' end. Are there any suggestions for the most efficient way to do this? Is the best option to PCR with a...

04 May 2015 1,698 9 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View