Stephanie S Coster

6 Questions 9 Answers 0 Followers

Questions related from Stephanie S Coster

Suggestions for AFLP kit?

I'm creating a lab for students to run AFLP reactions on an organism of their choice. I'm trying to source the supplies and have seen that there used to be an AFLP kit from Invitrogen (now...

06 June 2018 6,910 5 View

Can I use a hot-start Taq for pre-selective PCR in AFLP?

I'm trying to run a methylation-sensitive AFLP analysis and I only have hot-start taq (GoTaq). I've seen in other AFLP procedures that you should not use hot-start Taq for pre-selective PCR...

07 July 2017 6,658 4 View

Can I use a tabletop centrifuge (max 3000rpm) with Qiagen's DNeasy 96 Blood & Tissue kit?

I see in the Qiagen handbook that it recommends you buy their proprietary centrifuge in order to get high enough rpms (3000 & 6000) necessary for optimal DNA extractions. I have access to a...

07 July 2017 1,707 2 View

What does a "5% ramp down to 57C" mean?

The PCR protocol says after 15min at 95C, there was a 5% ramp down to 57C. I'm familiar with a touchdown PCR, but this terminology is new to me. Does this mean the annealing step starts at 95C and...

02 February 2016 9,683 3 View

Is there a tool to help design primers for single base extension?

I'm trying to design primers for analyzing SNPs via single base extension. I know I need to use a F or R primer that lines up to the SNP, but I'm trying to figure out how many base pairs to use,...

09 September 2015 1,920 3 View

Can you give any tips for designing primers when there is no flanking region?

We are trying to design primers for SNPs that have little to no flanking region on the 5' end. Are there any suggestions for the most efficient way to do this? Is the best option to PCR with a...

05 May 2015 1,749 9 View