05 October 2020 4 2K Report

These methods are used to solve linear system of equations.

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Can methyl cellulose be used as a binder in the preparation of zeolite pellets?

I am currently preparing zeolite pellets for adsorption applications, specifically for removing phosphate and ammonium from aqueous solutions. So, I need to know about the binder used during this.

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What is the Optimum Neon Transfection Setting for CRISPR/Cas9 RNP in THP-1 Cell Line?

I am trying to transfect THP-1 cells with RNP of CRISPR/Cas9-GFP using ThermoFischer Scientific Neon Transfection System. The current settings are 1400V, 20ms breadth, and 2 pulses. I have done it...

26 May 2024 970 0 View

How do I prepare zeolite pellets that can use for metal removal and how can i prepare different size if pellets?

I want to remove phosphate and ammonium from wastewater for this I want to prepare zeolite pellets.

15 May 2024 3,342 0 View

How to attach 6-thioguanine with citrate stabilised gold nanoparticles?

We have prepared 14 nm GNPs by using classic citrate synthesis method where trisodium citrate is being used as reducing and stabilising agent. Then we put 6-thioguanine (purine analogue with an S...

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What causes smears in electrophoresis results of nested PCR products and how to overcome it in PCR process ?

On electroforesis result, there was a spesific band but always have smears on it

26 September 2023 10,007 1 View

The value of my adsorption energy for a gas adsorbed on a metal oxide surface is positive. Is it acceptable? How do I explain it?

I am working on the adsorption properties of formaldehyde on a metal oxide monolayer. The least adsorption energy value for one of the configurations turned out to be 1.55 eV. What shall I do now?

14 August 2023 5,715 1 View

Tetra-Arms PCR: Gel shows CC and CA alleles but no AA alleles in any sample. Technical issue or probability?

40 samples show one heterozygous (CA)(175bp, 202bp) and one homozygous allele (CC)(202bp) only and not (AA)(175bp) allele. Attached is just a snippet of some samples showing CA and CC allele...

04 July 2023 5,698 1 View

Smart-Pls ? , Discriminant validity , What is the recommendation ?

Can we confirm discriminant validity for the measurement model in the following case? AVE for latent constructs with the correlations among measurement constructs strongly supports (Fornell &...

13 June 2023 8,399 0 View

How to store ultra-centrifugal Amicon filters for further usage?

I have used my amicon filter one time for concentrating down the TEV protease. I want to re-use the device again in future for the same protein. How to store? Buffer and storage temperature?

12 June 2023 2,774 3 View

Tetra ARMS PCR shows only one mutation in samples. How to troubleshoot to get the other when I don’t have any Positive control?

My Outer forward, Outer Reverse bp size is 326bp, which shows in my samples. There are two possible mutations: One showing band size of 202 and the other at 175. All of my samples show either no...

08 June 2023 237 7 View

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