i am planning to study the role of sucrose in invitro germination of various AM fungal species
In general, sucrose gives energy to fungi, bacteria and seeds for germination and growth,
and I think your results could add extra information on this topic.
Sucrose is a source of carbon which the roots require for growth. Without sucrose in the medium, the transformed roots will not grow. This is a well known fact. However, in vitro germination of AM spores is best in the medium that is devoid of sucrose or any other source of carbon.
I believe moisture/water is necessary for initial germination of spores including AM. Even the water-clerigel plate, should also promote the initial AM germination. However Sucrose can be added to make sure the checking of contamination.
AM spores germination can take place even on plain agar. Studies have shown that addition of sucrose has resulted in poor AM spore germination. In what way sucrose can check contamination?
I mean contamination:
Generally when AM spores isolated from the soil followed by disinfection treatment for invitro germination, at that time if germination is done in non sucrose media, the heterotropic contamination (Bacteria or fungus) will not appear quick, instead if we use sucrose in media it can appear fast and can save time.
But I agree with Dr. Rodrigues,,..in present of C source the AM germination is poor.
1- can you explain more about your experience ??
Are you associated the AMF with transformed roots, What your objective exactly ?
2- Sucrose is a source of carbon of transformed roots as chicory and DC2 .....
is better to use medium liking sucrose in in vitro germination of AMF spores but with autotrophic plant
As mentioed earlier sucrose is the carbon source and for any function to work sucrose is an important signal,.
Dear James,
In my opinion, sucrose can play some part in AM spore germination as a signal for fungus because roots contain it, but not a nutritional one. As far as known, AM fungi metabolize hexoses and can utilize sucrose only after its cleavage by plant invertase. AM germinated spores are even more restricted in its metabolic capabilities than mycelium during symbiotic phase. The information on sucrose and AM fungi you can find in Smith& Read`s "Mycorrhizal Symbiosis", the chapter "4 – Growth and carbon economy of arbuscular mycorrhizal symbionts" http://www.sciencedirect.com/science/article/pii/B9780123705266500064 and more recent title "Molecular mycorrhizal symbiosis", Ed F Martin, 2016, the chapter 13 "Primary metabolism in arbuscular
mycorrhizal symbiosis: Carbon, nitrogen and sulfur" DOI: 10.1002/9781118951446.ch13
With best regards,
Elena
Dear James,
You may go through the reaserch publication Vilarino, A. (1997) Treatment of Glomus mosseae propagules with 50% sucrose increases spore germination and inoculum potential for some possible inputs.
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