Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

What is the first thing that comes to your mind when you think of the effectiveness of foreign aid on development? Effective/not effective, why?

I'm currently doing a research project on the effectiveness of U.S. aid in Georgia, and would very much appreciate your insights.

22 December 2020 4,189 1 View

Can peptides survive high temperatures?

I'm looking for ways to functionalise a material with a cell-adhesive peptide (relatively short chain - 6aa) and I was just wondering if peptides can withstand high temperatures (100-150 C) for 30...

15 January 2020 8,978 4 View

Abnormally high fluorescence read with qpcr, and no amplification showing on agarose gel, what could be going on?

Hi! I've been running qPCR on some drinking water samples with a primer/ probe FAM assay. In any given run, there have been at least a few samples with artificially high fluorescence reads,...

16 October 2019 8,060 3 View

How to prepare agar amended with mercuric chloride?

Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some...

17 June 2019 5,047 5 View

Interview guides for UTAUT?

30 January 2019 5,443 4 View

How much does our childhood affect the way we think and make investment choices?

Subconscious, subjective drivers.

22 January 2019 5,397 5 View

ReNcell VM cell culture problems - why are they dying after I split them?

The cells look healthy after thawing, but they die when I try to split them. I have tried making fresh media and fresh matrigel, and have tried filtered and non-filtered Accutase. Does anyone have...

19 December 2018 630 9 View

Is it necessary to dechorionate zebrafish embryos to extract genomic DNA and RNA ?

I tried to dechorionate embryos with pronase at 1mg/mL. I made 3 washes but I obtained a high mortality rate and the medium was dirty. Thank you for your answers

03 January 2017 3,455 4 View

Can you help me with Activity measure of trypsin?

Sustrate=BAPNA I get a white solution when i mixed BAPNA(with DMSO) and buffer Tris-HCL/CaCl2 First 3 minutes the activity goes down, after 3 min stars to go up but slowly. Is this normal?

25 July 2016 6,718 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View