01 January 1970 1 5K Report

I have been reading about various fluorescent proteins and most of the work seems to come from Japan. Are there any groups who would study, discover and/or improve fluorescent proteins based in Europe?

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Anybody with experience with whole plasmid sequencing? Should I worry about plasmid oligomers?

I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...

19 June 2024 1,280 5 View

What can be yellow colonies after E. coli TOP10 trnasformation?

Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...

16 June 2024 3,272 3 View

How to reduce the number of measurements/iterations needed in deep reinforcement learning?

I'm trying to use reinforcement learning with live EEG measurements. However, just 2000 measurements/iterations take 16.6 minutes to measure and it seems I need at least 10 hours of live...

12 June 2024 2,884 3 View

What can be wrong with this agarose gel?

A friend did PCR and then loaded it on agarose gel. All samples were prepared in the same way; the PCR was identical, with the same template, only primers differed, the same amount loaded on the...

04 June 2024 3,049 3 View

How can I see all points in a 5-95 percentile Boxplot in Graphpad?

Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...

17 April 2024 6,093 0 View

Why could amplicon sizes appear bigger than they should on agaose gel electrophoresis?

I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...

09 April 2024 4,487 5 View

What kind of diatom is this?

I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...

19 March 2024 1,763 10 View

Does somebody have experience with ThermoFisher's Platinum SuperFi II DNA Polymerase?

I just found the Platinum SuperFi II DNA Polymerase, which should simplify PCR protocol as it allows uniform annealing temperature of 60°C, but it should also have very high fidelity; >300×...

17 March 2024 7,686 4 View

Me puede proporcionar iso 9001:2015?

quisiera que me proporciones un sistema de gestion de calidad

15 March 2024 3,491 1 View

Iso 9001:2015?

ME PUEDE PROPORCIONAR INFORMACION SOBRESistema de Gestión de Calidad Basado en la Norma ISO 9001:2015 para el Instituto de Educación Superior Tecnológico

15 March 2024 447 0 View

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