What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

How can you alter line thickness in emmip plot (R)?

I am working with R and plotting emmeans graphs, yet I cannot find online how we can change the line type or its attributes using the emmip function. I have two independent categorical variables,...

27 October 2020 9,339 1 View

How to synchronize two stepper motors most optimally?

We need to push a large load (2 tones) using two stepper motors, connected rigidly to the axis (two screws). I'm worried the motors could micro-step out of sync. And cause malfunction. I can see...

21 October 2020 6,363 4 View

How to fix convergence problmes using Mohr Coulomb Plasticity?

Dear colleagues, I am trying to simulate a strip foundation on a cohesionless soil with a friction angle of 30 degrees. However, I obtain convergence problems. I am using a geostatic step. The...

21 October 2020 4,556 2 View

Can you integrate Zotero with this website?

Is it possible to integrate Zotero or any other bibliographic software application with this website application? If not, is it possible to do that with academia, ssrn or Google Scholar? Any...

21 October 2020 4,712 1 View

How to find out, if a recent Sentinel-2 acquisition failed or had a problem?

Hello, I am currently waiting for an image to appear on Scihub and it takes longer than usual (acquisition date 2020-09-22, orbit r122, T33UWR tile actually, but looking for general answer). So it...

23 September 2020 6,842 5 View

Can small law firms adopt AI?

After considerable research, I have found several use cases which can be used to exemplify some implementations of AI on law firms. However, there is clearly a disadvantage for this segment when...

01 September 2020 7,132 5 View

How can i model a shock tube with 2 different gases on Fluent?

Hi, I'm currently trying to study shock tube analysis on fluent and I have modelled a shock tube with a driver section (high-pressure) and a driven section (low pressure). What I want to study is...

22 July 2020 8,162 3 View

How can i make an isopeptide bond in GROMACS ?

Hi everyone I'm new in all the field of molecular dynamics, but i trying to run a SMD of one protein with two isopeptide bond, one in each domain, that prevent my protein stretch. My problem is...

07 July 2020 6,201 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View