How nessesary is working point zero charges for any adesorbent?

How nessesary is working point zero charges for any adesorbent? I want to know how suitable the surface of the material is good for adsorption. I got SEM and FTIR results and thought those are...

30 January 2021 5,120 2 View

What are the parameters for sustainable fibres for concrete?

28 June 2020 1,987 1 View

On what issues the performance of Neural Network mainly depends?

On what issues the performance of Neural Network mainly depends?

20 January 2019 4,434 8 View

Does the pre-induction OD600 affect expression of stable proteins from bacteria?

We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...

19 March 2018 767 2 View

I am able to detect DNA bands on agarose gel from negative controls of PCR products? Did any one comeacross the same problem?

Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...

29 August 2017 9,201 9 View

Why do HIV vaccine candidates developed so far fail to show good protective efficacy during preclinical and/or clinical trials?

It has been more than three decades since HIV/AIDS has already spread throughout all the five continents (North America, South America, Europe, Africa, Asia and Australia). A lot has been done to...

17 July 2017 709 6 View

Best software for amplicon seq data analysis for malaria parasite.

14 July 2017 2,025 1 View

Data analysis using qiime for malaria parasite clones

14 July 2017 4,585 2 View

Is it possible to engineer E. coli strains that do not produce endotoxins?

E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....

18 June 2017 9,754 11 View

Is it possible to exteract two exposure OR for one outcome from one study for meta analysis?

01 January 1970 8,239 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View