Streptomycin resistant mutants

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Electrochemical rhodococci

Hi, I have made a recombinat plasmid that contains a deleted gene, I want to electroporate this back into Rhodococcus sp. NCIMB 12038 wild type and wanted to know does any one have a protocol to...

11 December 2011 5,111 0 View

GC-MS extraction method

I am looking for a protocol or procedure to extract hydrocarbons from water contaminated by Diesel. At the moment I am trying with ethylacetate adding the double Volume of this compound to the...

09 October 2011 4,840 2 View

GC-MS extraction method

I am looking for a protocol or procedure to extract hydrocarbons from water contaminated by Diesel. At the moment I am trying with ethylacetate adding the double Volume of this compound to the...

09 October 2011 5,393 3 View

Blue/white screening problem

I have cloned a 915bp gene into puc19, I have deleted the central protion from this gene using restriction digestion,purifed and recircularied my recombinant vector. I then isolated my deleted...

02 March 2011 1,766 0 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Basak Ozturk

Hhmm... Open a plate under the clean bench with the UV light on for a couple of minutes?

Alternatively you may try to isolate mutants with increasing Sm concentrations, sub-inhibitory to MIC and then increasing to perhaps double the MIC -may produce more reliable results.

Norbert Ponelies

Hi Aaron,

maybe is Pup help you:

http://www.scielo.br/scielo.php?pid=S1517-83822008000400017&script=sci_arttext&tlng=en

Nice day np