Blue/white screening problem

03 March 2011 0 2K Report

I have cloned a 915bp gene into puc19, I have deleted the central protion from this gene using restriction digestion,purifed and recircularied my recombinant vector. I then isolated my deleted gene by retsriction digestion and cloned into another vector pk198mobsacB, which can use blue white screening, I cloned my deleted gene (500bp) into this new vector,however i am screening using x-gal and all i am getting is blue colonies.

is it possible that my deleted gene has not disrupted the open reading frame entirely alllowing the production of b-galactosidase?

can anyone help?

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