Electrochemical rhodococci

12 December 2011 0 5K Report

Hi,

I have made a recombinat plasmid that contains a deleted gene, I want to electroporate this back into Rhodococcus sp. NCIMB 12038 wild type and wanted to know does any one have a protocol to make electrocompetent rhodoccci and the paramaters i need to use to electroporate my dna into the cells.

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