I've been isolating marine Streptomyces for about 15 yrs, but just switched to soil Strep. I typically use oatmeal agar to get these microbes to sporulate. I make spore stocks then for storage. Does anyone know if adding MgCl2 or CaCl2 to the media will help with the extra hard ones? I've tried ISP2, ISP4, oatmeal, R2YE and sporulation agar, but in some cases the microbes won't sporulate on any of those. I've read somewhere in the past that you can use either of them to revive a culture from a slant. If you have any media ideas I could try that would be greatly appreciated as well.
Low phosphate/high calcium should do the trick. I also read that soil extracts and/or lignocellulose strips are used to initiate sporulation, also some species sporulate on chitin. Good luck!
You can use Fish Meal Agar for sporulation. Try also starch nitrate agar. Yes try to add a little bet more MgCl or MnCl.
At times I have observed getting good growth of Actinomycetes colonies on SDA with Chloramphenicol. Just try once in this aspect.
Like Jakub I have also read that soil extract agar induces sporulation.
Did you ever get them to sporulate? I am trying with Streptomyces 85E on ISP4, from the last -20C stocks that we have stored, unfortunately the stocks are quite old and I know that -20C often reduces viability, it used to work but now they are not even growing at all, guess the stocks are not viable. I have some old plates I tried this with but I can only get hyphae growth, no sporulation.
Anyone have any tips?
Thanks!
Try with oat meal broth supplemented with traces of some minerals such as Mn , Mg and Nacl.
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