Is antigen retrieval step required for frozen spinal cord sections in IHC ?

I am doing co-immunostain using frozen spinal cord sections for IHC. Is it required to perform the antigen retrieval step prior to the blocking step ?

20 August 2020 4,144 0 View

How to calculate mutual information and joint entropy for the following datasets?

I have the following data set (attached) and I would like to calculate mutual information and joint entropy between multiple columns (like for A,B,D,E or C,D,E,F,G etc.). I have gone through R...

29 July 2020 2,863 2 View

Which variables do not have zero value?

Which variables do not have zero value? Also give an example of multiple power regression.

18 July 2020 6,527 3 View

What is the existential significance of English in Nation Building?

Whether the role of Language is really underrated in Nation Building?

25 February 2020 5,116 15 View

Why does the tip a Ag/AgCl electrode become blue during storage in a KCl solution?

We are using Ag/AgCl electrode for studying supercapcitors of polyaniline based working electrode. The working voltage range is 0 to 1 V. During the storage of reference electrode, it is observed...

07 October 2019 6,812 3 View

How do I learn to do in silico analysis?

I want to see the change in binding ability of the enzyme with the substrate in presence of a probable drug molecule insilico. Where can I find reliable information to learn about the basics from...

01 February 2019 7,430 4 View

How metal NW array and transparent conducting films are different when one compares the NIR transmission spectra?

Due to high electron concentration in a metal or transparent conducting film the NIR transmission falls sharply due to free carrier scattering or plasmon resonance.However, in case of metal NW...

17 September 2018 3,181 3 View

Why Cu, Al and Mg are most used X-Ray anode??

In most of the XRD instrument Cu is used and in most of the XPS instrument Al/Mg is used? Is there any specific reason behind this??

05 August 2018 1,825 4 View

Wll there be any problem to use a magnetic target to do RF/DC sputtering??

I would like to deposit film of Yttrium iron garnet using magnetron sputtering technique. Will there be any problem during deposition?? Any risk to the system??

02 August 2018 7,051 14 View

Can anyone give an estimate on the market research on the worth of ZnO based TCFs?

I would like to know how much ZnO-based TCF (AZO, GZO) contribute (in % or in US$) to the present TCF market and any future forecast??

07 July 2018 1,226 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View