I am looking for alternative of Filipin staining for sterols which have higher or lower affinity to specific kind of sterols.
Thanks
In the InderScience submission, I need the names and details of 4 international experts (I am from India, so at least 2 must be from outside country) I am trying to submit an article that is based...
11 March 2024 9,402 0 View
How to select bed material along with flow during the modeling of local scour around the bridge pier in ANSYS FLUENT?? Please provide some sources to tackle this issue.
26 January 2023 9,848 2 View
Hello I am doing 5kb cloning. But unfortunately I am getting band in 3kb,can anyone tell me what is the exact reason to get at 3kb band? I have made primer in 4 fragment (approximately 1.25kb for...
07 June 2022 6,900 0 View
Hey, I am using 96 well plates to grow Bacteroides samples and tracking their OD using an automated plate reader. I have to take readings for a long period of time (48 or 72h) and so am...
04 January 2022 581 2 View
Hello, I am trying to purify a recombinant his-tagged protein (MW: 29.5 kd, pI:5.7, mostly composed of beta strands). It has been expressed from BL21(DE3) competent cell. I am using 50 mM Tris,...
14 December 2021 1,077 5 View
i know how to carry out Poisson regression for count variable such as total number of children in spss but not modified Poisson regression
27 May 2020 6,044 0 View
Specially for different sectors different Training and development activities will boost up economic crisis. even those who lost their job like garments workers how we can utilise them by...
20 May 2020 1,991 3 View
I carry out biosensing using thin film gold electrodes. I measure the impedance magnitude and phase as a funtion of frequency. Will plotting |Z| vs θ be similar to Z' vs Z" (real impedance vs...
25 March 2020 1,226 4 View
Greetings! I am currently working with a kinase name Microtubule affinity regulating kinase 2( MARK2). Every time I purified this protein, I have never got a good yield. Usually after...
12 February 2020 1,798 2 View
Greetings! I am now working on a F-moc based peptide synthesis having following sequence: GFARVYMSRSSGWERPSGGNVKCKIGSTENLK (N terminal to C-terminal) For the cleavage/micro-cleavge, I need to...
09 February 2020 6,467 6 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View