How Training and Development help reshaping economy after pandemic situation?

Specially for different sectors different Training and development activities will boost up economic crisis. even those who lost their job like garments workers how we can utilise them by...

20 May 2020 1,867 3 View

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25 March 2020 1,130 4 View

I am loosing all my protein when I try to concentrate it. Can you please suggest me a anything about it?

Greetings! I am currently working with a kinase name Microtubule affinity regulating kinase 2( MARK2). Every time I purified this protein, I have never got a good yield. Usually after...

12 February 2020 1,698 2 View

Could somenoen help me with three point flexure modelling of hollow composite beams in LS DYNA?

I am trying to model quasi static three point flexure of hollow circular composite beam in LS DYNA. I am using MAT 58 and B-T shell elements. The beam undergoes progressive loading in an expected...

01 June 2019 2,884 1 View

What study design should I use for Retrospectively comparing 2 drugs for their efficacy and safety ?

Planning to do retrospective chart analysis to compare 2 drugs for a common medical condition in terms of there efficacy and safety. What designs should I use, how to decide sample size and what...

11 December 2018 2,616 4 View

How can I protect thin film (30nm) gold electrodes from electrolysis?

I apply voltage (AC, DC and pulse) to biological cell monolayers using 30nm thick gold electrodes. My observations are significantly affected by electrolysis (formation of gas bubbles). Moreover,...

18 February 2018 3,747 5 View

When electrolysis occurs, does the anode or cathode heat up more (or by same extent)?

Does it depend on the relative size of the electrodes?

31 January 2018 9,866 3 View

When doing biological cell migration, can electrode be connected directly to agar bridge, or does it need to be immersed in salt solution?

Does the electrode need to be of a specific metal? Which salt solution and concentration to use?

25 October 2017 6,914 1 View

Is there a reliable way of "visualising" an electric field between 2 planar electrodes?

My electrodes are 2 bands of gold with a gap in between || Is there any way of making sure an electric field exists between the electrodes?

19 October 2016 3,568 5 View

How to simulate contact between flat surfaces when they are at an angle with respect to one another?

22 September 2016 2,399 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View