Stem cells tracking in vivo

More Angela d'Esposito's questions See All

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

Why I am not getting nice bands in my Nested PCR?

I am running a Nested PCR on blood DNA. Although the primers have worked successfully in previous publications, I am not getting a clear bands for sequencing. The first PCR reaction mix...

25 April 2024 4,661 3 View

Are there risks associated with the routine laboratory use of Sysmex DI-60? If so, what are they and how significant is its impact in the laboratory?

With the recent developments on automation in the laboratory, it has recently produced huge impacts in the laboratory workflow we experience today. Based on several studies, its increased...

11 April 2024 544 0 View

How does co-infection with hepatitis B virus (HBV) affect the diagnosis of HDV infection?

In relation to an article titled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan et al. (2023)

08 April 2024 4,432 1 View

What pre-analytical variables contribute to variations in LDH activity measurements in underfilled tubes?

The preanalytical phase, which presents one of the biggest obstacles to laboratory workers, is also the most susceptible element of the whole testing procedure.There are a number of requirements...

07 April 2024 8,207 0 View

Can I get materials on African Decolonization?

Decolonization on African history and material cultures.

20 March 2024 9,812 1 View

EU's affect on Apple's Business?

28 February 2024 6,284 2 View

What are topic ideas for leadership dissertation?

My field of study is Doctor of Management. I currently work in the medical field as a medical records specialist. I need help coming up with a topic to write on. Any suggestions and or ideas...

06 December 2023 7,789 1 View

How can I exchange the 2023 Gender & STEM Article (Schorr & Gorovoj) by a new version?

This article was falsely (automatically, not by me!) categorized as a chapter of a book because the Icon of the Hill Publisher was printed on the first page. But it is an article in the Journal of...

06 December 2023 4,067 1 View

Does anyone know what could be going wrong for gDNA amplification using qPCR not to happen?

I am using 4 set of primers to quantify 4 bacteria, those primers were designed to amplify gDNA from each one of that bacteria. I performed lots of assays and everything went well, until one month...

28 November 2023 4,467 2 View

Problems about acousto-optic coupling in Comsol, how to check whether the moving mesh is correct?

I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...

13 August 2024 9,795 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

abdulMajeed alwan Hammadi

it is really one of the methods that can give conclusions regarding end fate and differentiation issues i do not know methods other than isotope combination

Saleh Alkarim

Hello

Kindly look at links here

Good Luck

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888495/

https://www.intechopen.com/books/stem-cells-in-clinic-and-research/clinical-stem-cell-imaging-and-in-vivo-tracking

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-016-0396-y

https://jcmr-online.biomedcentral.com/articles/10.1186/1532-429X-16-7

Angela d'Esposito

Great, very useful, thank you very much!