Dear all,

I treated wild-type cells and knockout cells with two drugs X and Y for 24 hours. Then I measured the Gene A expression. Drug Y significantly increased Gene A expression around 500-fold only in WT cells. Also, Gene A expression is really low (~10%) in KO cells compared to WT cells. Please see the image.

My very basic understanding of statistical methods for this is a two-way ANOVA since I have two independent variables, the cell type and drug treatments. However, the issue is that these huge changes make the data fail to pass Levene's test for homogeneity as well as Shapiro's test for normality. A two-way ANOVA returns weird results and seems not suitable.

What overall tests should I perform, and what post hoc analysis should I follow?

Thank you in advance!