How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Can anyone direct me to a paper or chapter that discusses calculating sample size in a self-selecting survey study?

Our study is investigating clinical knowledge in a specific area in healthcare workers at a large metropolitan hospital. Participants will self-select to complete the survey via email invitation...

11 January 2021 7,934 2 View

Align list of short sequences to miRBase?

I have a list of short sequences from miRNA sequencing in an Excel format and want to align these to miRBase. How can I do this? Is there a function in R? I converted the Excel list into a fasta...

08 September 2020 9,908 3 View

How to get return loss below -10dB for monopole antenna which has very less or no ground plane (or directly interfaced with sma connector) ?

I am designing a monopole antenna For 59MHz frequency . The lamda length is 5m. I tried for lamda/2 which is directly interfaced to sma connector. Couldn't get proper return loss.( According to...

11 March 2020 4,885 2 View

Can anyone recommend a stain to identify mast cells/eosinophilic granular cells (EGC) in fish?

Hi, I was wondering if someone can recommend a stain where fish EGCs or mast cells show significant metachromasia. Toluidine blue did not work in my fish species. I did find recommendations on...

04 March 2020 7,817 1 View

Alpha/betha TCR detection in naive TCs without activation?

Hello together, I want to see differences in TCR expression in naive CD4/CD8 TCs. The problem is, if I would stain the TCR with soluble antibodies to check via FACS, this would lead to a weak...

23 November 2019 1,430 1 View

Questions about RNA extraction?

Hello, I have a problem with my gene expression results. The results of my endogenous gene are oscillating between samples, generating false negative results. It takes about 12 hours after sample...

08 October 2019 569 3 View

Why did I get RMSD plot with 10ns if the MD was 100ns trajectory?

I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...

04 September 2019 3,669 6 View

Doubt about secondary antibody to immunohistochemistry?

I bought a secondary antibody to use for immunohistochemistry, but the manufacturer says the antibody is only validated for Western Blotting, not Immuno. Because of this, I don't think which...

26 August 2019 5,356 7 View

High background on stain free blot after transfer and its complications for protein normalization(TGX Stainfree Biorad Gel)?

Hey, I have a question regarding the stain free blot technology of Biorad. After electroforesis I take a picture ("Stain-free gel") for activation of the trihalo-compound. Next I transfer the gel...

12 August 2019 7,734 8 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View